Journal: Scientific Reports
Article Title: Integrated, automated maintenance, expansion and differentiation of 2D and 3D patient-derived cellular models for high throughput drug screening
doi: 10.1038/s41598-021-81129-3
Figure Lengend Snippet: Schematic of the automated platform. ( 1 ) Liquid handler Biomek NX Span-8 including the plate shaker Shaking Peltier ALP and a tilting station Amplius (AIG) 3D Tilting ALP, ( 2 ) Liquid handler Biomek FXP (96- and 384-pipetting head including active wash-station and a BioShake 3000, ( 3 ) Acoustic droplet ejector Labcyte Echo550, ( 4 ) High content imager Yokogawa CV8000, ( 5 ) plate sealer Wasp, ( 6 ) Cell counter Vi-Cell XR12, ( 7 ) plate seal remover Nexus Xpeel, ( 8 ) Barcode printer and reader Scinomix Sci-Print MP2, ( 9 ) Precise SCARA robotic arm on rail, ( 10 ) 4 °C and 37 °C incubators Cytomat 24-C, ( 11 ) Sigma refrigerated centrifuge 6-16KR, ( 12 ) Tube de-capper Cap-it All, ( 13 ) Plate washer Biotek 405 LSHTV, ( 14 ) Multimode plate reader SpectraMax i3 Multi-Mode Platform and Minimax imager, ( 15 ) Custom made biosafety class 2 enclosure, ( 16 ) 2D-barcode reader Visionmate, ( 17 ) 4 deliiding modules, ( 18 ) 5 Tipbox labware feeders, ( 19 ) Regrip station, ( 20 ) 4 shelf labware hotel.
Article Snippet: Further devices for compound and plate management are the Wasp plate sealer (kbiosytems), the plate seal removal system Nexus Xpeel (Brooks life sciences), the barcode printer/reader Sci-Print MP2 (Scinomix), the tube (de-)capping device Capit-All (ThermoFisher), the 2D code reader VisionMate (ThermoFisher), the plate washer 405 LSHTV (Biotek), and a regrip position next to the Scara robot rail.
Techniques: